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Updated: Sep 10, 2025

Measuring the Kinetics of mRNA Transcription in Single Living Cells
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无细胞转录-翻译与等离子体交叉的数学模型

Yue Han1, Alexandra T Patterson1, Fernanda Piorino1,2

  • 1School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, United States.

Synthetic biology (Oxford, England)
|August 27, 2025
PubMed
概括

细胞自由表达系统可能会受到等离子体交互的影响,其中基因表达水平相互作用. 一个新的模型解释了这些相互作用, 改进了合成生物学工程和生物传感器设计.

关键词:
没有细胞的系统基因表达数学建模合成生物学系统生物学

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The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
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Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
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RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
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