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使用不同的阴性聚合物对有效的pDNA输送脂质复合物的影响

Giulia Anderluzzi¹, Tasnim Mohamed², Giorgia Moschetti¹

¹Department of Pharmaceutical Sciences, University of Milan, Milan, Italy.

International journal of nanomedicine

|August 27, 2025

概括

脂多复合体 (LPP) 提供了改进的基因传递. 聚乙烯胺 (PEI) LPP表现最好,显示出低毒性和高传染效率的等离子体DNA传递.

科学领域:

生物材料科学
纳米技术
基因治疗

背景情况:

脂多复合体 (LPP) 是先进的非病毒基因传递载体.
它们旨在通过降低毒性和增强传染来改善早期的多重复合和脂质复合.
尽管LPP具有潜力,但仍未得到充分探索.

研究的目的:

研究LPP成分对物理化学性质的影响.
评估LPP的细胞毒性和生物活性,包括细胞吸收,内体逃生和转染效率.

主要方法:

使用微流体方法制备LPP,将阴离子聚合物 (素,聚-L-素,PEI) 与等离子体DNA和脂质体结合起来.
进行了聚合物/DNA/脂质比率和微流体参数的优化.
其中包括尺寸,PDI,zeta潜力,FRET,SAXS和冷EM.

主要成果:

优化的LPP具有~180nm直径,PDI<0.2和中性/轻微阳性泽塔电位.
聚合物类型影响了三元复合结构.
与多重复和DOTAP脂质复合体相比,LPPs在体外具有较低的毒性和更高的蛋白质表达.
基于PEI的LPP显示出最高的内分离活性和转染效率.

结论:

LPP代表了塑体DNA输送的一个有前途的平台.
基于PEI的LPP是最有效的配方.
性聚合物选择对纳米粒子运输和基因传递有效性产生重大影响.

关键词:

提供DNA 基托桑的使用脂多复合体微流体聚L-氨酸聚乙烯胺

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