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Updated: May 5, 2026

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通过数字PCR快速准确地量化微卫星中的双链断裂

Cécile Palao1,2,3, Adèle Kovacs1, Maria Teresa Teixeira3

  • 1Département Génomes & Génétique, Institut Pasteur, Université de Paris, CNRS UMR 3525, 25 rue du Dr Roux, 75015, Paris, France.

Biology methods & protocols
|August 27, 2025
PubMed
概括

数字PCR (dPCR) 精确量化重复DNA中的双链断裂 (DSB),性能优于其他方法. 这一突破有助于诊断遗传疾病和了解癌症的发展.

关键词:
DNA 双链断裂数字PCR检测脆弱的地方非B型DNA结构重复扩张的障碍

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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