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Updated: May 11, 2026

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

Published on: November 27, 2019

基于生物信息学的糖尿病足中中性细胞外生物标记物的鉴定

Kang Liu¹, Lei Lei¹, Xin-Lei Yang¹

¹Department of Burn and Plastic Surgery, Affiliated Chenggong Hospital of Xiamen University, Xiamen, Fujian, 361001, People's Republic of China.

Journal of inflammation research

|August 27, 2025

概括

中性粒细胞外陷 (NET) 在糖尿病足 (DFU) 中至关重要. 这项研究确定了S100A12和HPSE为关键生物标志物,为DFU提供了新的诊断和治疗见解.

科学领域:

免疫学
遗传学
糖尿病学

背景情况:

中性细胞外陷 (NETs) 在糖尿病足 (DFU) 发病过程中发挥作用.
针对与NETs相关的基因 (NETRGs) 为DFU提供了潜在的治疗策略.

研究的目的:

确定并验证关键的NETRG作为DFU的生物标志物.
研究NETRG在DFU发育和免疫细胞透中的作用.
根据已识别的生物标志物开发一个DFU的诊断模型.

主要方法:

差异性基因表达分析以确定DFU患者的NETRG.
机器学习算法来构建名录并识别生物标志物.
免疫透分析和生物标志物表达的qRT-PCR验证

主要成果:

在DFU中,S100A12和HPSE被确定为显著上调的生物标志物.
开发了一种结合这两种生物标志物的诊断模型.
在DFU中观察到T辅助细胞和中性粒细胞的比例增加以及激活B细胞的减少.

结论:

NETRGs,特别是S100A12和HPSE,在DFU发展中发挥着重要作用.
这些生物标志物为改善DFU的临床诊断和治疗策略提供了潜力.
了解NETRG机制为DFU病变提供了新的见解.

关键词:

糖尿病足部诊断情况一个功能免疫透一个机制与中性细胞外陷相关的基因

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Glucose Transporters

Glucose Transporters

Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes: