Porphyromonas gingivalis 劫持 mitophagy 和 lysosomal 功能 在内皮细胞中持续存在
Cheng Zheng1, Jianmin Huang1, Shengming Xu1
1Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Frontiers in cellular and infection microbiology
|August 27, 2025
概括
Porphyromonas gingivalis 劫持了细胞和损害了内皮细胞中的 lysosomal 功能. 这一过程促进了细菌的存活,并可能导致动脉样硬化的发展.
科学领域:
- 细胞生物学
- 微生物学
- 病变发生
背景情况:
- Porphyromonas gingivalis (P. gingivalis) 是牙周病的关键病原体,与动脉样硬化有关.
- 虽然非选择性自有助于P. gingivalis在内皮细胞中的存活,但选择性自的作用尚不清楚.
- 这项研究探讨了P. gingivalis对细胞和溶酶体进行细胞内持久性的操纵.
研究的目的:
- 调查P. gingivalis是否会劫持 mitophagy 和 lysosomes 在内皮细胞内持续存在.
- 在选择性自的背景下阐明P. gingivalis细胞内生存的机制.
主要方法:
- 人类大动脉内皮细胞 (HAEC) 感染P. gingivalis.
- 使用西式斑点,免疫光和电子显微镜分析菌.
- 通过阿克里丁色染色,溶解感应染色和西部斑点染色来评估溶解体功能.
- 使用抗生素保护试验和SYTO-9染色的P. gingivalis存活率的评估.
主要成果:
- P. gingivalis 触发了PINK1-帕金基介导的线粒,破坏了自/线粒流.
- 观察到溶酶体功能障碍,包括溶酶体数量增加和酸度下降.
- 通过启动线粒细胞和 lysosomal 功能受损,增强了 P. gingivalis 的存活率.
- 线粒细胞活化抑制了外细胞的形成和与溶酶体的融合.
结论:
- 在内皮细胞中,P. gingivalis通过启动细胞和损害 lysosomal 功能来促进其生存.
- 这种机制抑制了异菌体的形成和降解,有助于细菌的持久性.
- 这些发现提供了关于P. gingivalis病变及其在动脉样硬化中的作用的见解.
相关概念视频
01:17Phagocytosis of Apoptotic Cells
4.0K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
4.0K
01:36Delivery Pathways to the Lysosome
7.1K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
7.1K
00:41Phagocytosis
6.6K
Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis ("cellular eating") is one of three major types of endocytosis. Cells use phagocytosis to take in large objects, such as other cells (or their debris), bacteria, and even viruses.
The objective of phagocytosis is often destruction. Cells use phagocytosis to eliminate unwelcome visitors, like pathogens (e.g., viruses and bacteria). Many immune system cells,...
The objective of phagocytosis is often destruction. Cells use phagocytosis to eliminate unwelcome visitors, like pathogens (e.g., viruses and bacteria). Many immune system cells,...
6.6K
01:27Autophagy
4.6K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.6K
01:20Receptor-mediated Endocytosis
6.4K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
6.4K
00:58Recycling Endosomes and Transcytosis
2.8K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.8K