用于治疗标和癌症生物标记物识别LncRNA相关蛋白的方法
Mohammad Shabir Hussain1,2, Puneet Vij3, Sudhir Kotnala1,2
1Medicine and Oncology ISU, School of Medicine, The University of Texas Rio Grande Valley, McAllen, TX 78504, USA.
概括
长非编码RNAs (lncRNAs) 通过与蛋白质相互作用来调节癌症中的基因表达. 像LC-MS这样的蛋白质组技术可以识别这些相互作用,揭示癌症生物标志物和治疗点.
科学领域:
- 癌症学
- 分子生物学
- 蛋白质组学
背景情况:
- 长非编码RNAs (lncRNAs) 是癌症中基因表达和细胞信号的关键调节者.
- lncRNA功能通过与蛋白质合作伙伴的相互作用进行介导,影响染色体结构,表观遗传学,转录和信号传导.
研究的目的:
- 对 lncRNA相关蛋白质的蛋白质特征方法进行审查.
- 强调高通量液体染色体质谱 (LC-MS) 技术用于识别癌症特异性蛋白质特征.
主要方法:
- 基于亲和性的方法:RNA下拉,ChIRPMS,RAPMS,BioIDMS,SILACMS.
- 高通量LC-MS工作流程,包括消化,纳米LC分离和协同MS分析.
- 无标签量化,化,交叉链接和生物信息分析用于网络解决和验证.
主要成果:
- 鉴定 lncRNA-蛋白质复合体和癌症特异性蛋白质特征.
- 发现转化后的修改和对瘤生物学的机制性见解.
- 对肝细胞癌,结肠直肠癌和前列腺癌的潜在生物标志物的鉴定.
结论:
- 与lncRNA相关的蛋白质的蛋白质特征提供了临床可行的生物标志物和治疗点.
- 这些研究具有翻译意义,应该在癌症研究中得到更广泛的采用.
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