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ZmMYB104通过激活玉米中的ZmCAT2表达增强了耐热压力

Hao Zhang¹, Qiyue Wang^1,2, Teng Zhou¹

¹State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, College of Agronomy, Henan Agricultural University, Zhengzhou, China.

Physiologia plantarum

|August 27, 2025

概括

玉米植物通过ZmMYB104转录因子获得耐热性,该转录因子激活ZmCAT2基因. 这种机制增强了过氧化的吸收,减少了氧化损伤,提高了对热应激的抗性.

科学领域:

植物分子生物学
植物生理学
遗传学

背景情况:

温度的波动对植物的生长和发育产生重大影响.
了解玉米 (Zea mays L.) 等作物的耐热机制对于粮食安全至关重要.
玉米中耐热性的分子基础需要进一步阐明.

研究的目的:

研究MYB转录因子ZmMYB104对玉米热耐性的作用.
在热应激下识别ZmMYB104调节的下游目标基因.
描述ZmMYB104介导的热应激反应调节途径.

主要方法:

ZmMYB104的分子克隆和表达分析.
细胞下定位和交换活化试验.
通过RNA测序和DNA亲和性净化测序 (DAP-seq) 来识别目标基因.
电泳移动转移测定 (EMSA) 和双露西法酶报告测定以确认ZmMYB104-ZmCAT2的相互作用和转录活性.

主要成果:

在玉米幼苗中,ZmMYB104的表达是由热引起的,并作为核中的转录激活剂起作用.
过度表达ZmMYB104增强了玉米幼苗对热应激的抵抗力.
ZmCAT2被确定为ZmMYB104的直接下游目标.
ZmMYB104-ZmCAT2调节模块增强了过氧化 (H2O2) 清除能力,减少了热应激下反应性氧物种 (ROS) 的积累和氧化损伤.

结论:

通过直接激活ZmCAT2的转录,ZmMYB104赋予了热耐受性,这是ROS解毒中的关键酶.
ZmMYB104-ZmCAT2调节模块是谷物作物的热应激反应的一个新组成部分.
这一监管轴为通过分子育种开发适应气候变化的玉米品种提供了潜在的遗传目标.

关键词:

在CAT2 H2O2 其他 MYB转录因子热应力玉米

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