Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

620
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
620
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

556
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
556
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

934
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
934
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.8K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.8K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

889
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
889
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

1.1K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
1.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Direct Analysis in Real Time Triple Quadrupole Mass Spectrometry for Illicit Drug Identification and Quantification.

Journal of the American Society for Mass Spectrometry·2026
Same author

Building In-House Libraries to Use with the NIST/NIJ DART-MS Data Interpretation Tool.

Journal of the American Society for Mass Spectrometry·2025
Same author

Detection of anticoagulant rodenticides by direct analysis in real time time-of-flight mass spectrometry: novel screening techniques and rapid semi-quantitative determination.

Analytical methods : advancing methods and applications·2025
Same author

A Molecular Fragment Database Generated through Simulated Sequential Single-Bond-Breaking.

Journal of the American Society for Mass Spectrometry·2025
Same author

Enantiomeric Determination of Medetomidine in Street Drug Samples (August 2024-February 2025) and Implications for Immunoassay Test Strip Analysis.

Drug testing and analysis·2025
Same author

Quetiapine: A Novel Additive to the Illicit Heroin Supply in Los Angeles.

Journal of addiction medicine·2025
Same journal

Automated Pixel-Wise Recalibration for Improving Mass Accuracy and Peak Assignment in MALDI Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
Same journal

How "Soft" Are Your Gas Mixtures? Effects of Modifier Gas Types on the Dissociation of Labile Ions in Differential Mobility Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

A Robotic Sample Handling Platform for Fully Automated Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Direct Analysis in Real-Time Tandem Mass Spectrometry for Rapid Screening of Thirty-one Plant Growth Regulator Residues in <i>Rehmannia glutinosa</i>.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Characterization of Alkane Oxidation Products in a Corona-Discharge Reactor Using Ammonia-Doped Ion Mobility-Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Integration of a Modified Synchrotron Radiation Photoionization Time-of-Flight Mass Spectrometer with a Residual Gas Analyzer for Complementary Detection of Catalytic Products with Different Ionization Energies.

Journal of the American Society for Mass Spectrometry·2026
查看所有相关文章

相关实验视频

Updated: Sep 10, 2025

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.4K

采用环境电离质谱法捕获当前实践并描述被查获药物分析的测量可重现性:一项实验室间研究

Edward Sisco1, Dennis D Leber1, Arun S Moorthy2

  • 1National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States.

Journal of the American Society for Mass Spectrometry
|August 27, 2025
PubMed
概括
此摘要是机器生成的。

环境电离质谱 (AI-MS) 显示在被查获的药物分析中具有很高的可重现性,尽管方法各异. 标准化参数可以进一步提高实验室的质谱数据的一致性.

关键词:
在 ASAP-MS环境电离质谱一个 DART-MS实验室间研究查获的毒品频谱可复制性

更多相关视频

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

9.8K
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.5K

相关实验视频

Last Updated: Sep 10, 2025

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.4K
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

9.8K
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.5K

科学领域:

  • 法医科学
  • 分析化学
  • 质谱学

背景情况:

  • 环境电离质谱 (AI-MS) 越来越多地用于查获的毒品证据的初步选.
  • 与传统的GC-MS不同,AI-MS在电离源和质谱仪中提供了灵活性.
  • 仪器配置的变化可能会对AI-MS光谱数据产生重大影响.

研究的目的:

  • 在不同实验室和方法中评估AI-MS数据的可复制性.
  • 识别导致AI-MS数据变化的因素.
  • 评估标准化方法对AI-MS数据可复制性的影响.

主要方法:

  • 一项涉及来自17个实验室的35名参与者的实验室间研究.
  • 使用参与者的常规AI-MS方法分析了21个解决方案.
  • 使用等号相似度对质谱进行比较,以评估可重现性.
  • 一组参与者使用规定的方法参数分析样本.

主要成果:

  • 在各种样本类型,仪器,方法和操作人员中观察到通常高质谱可重现性.
  • 低碎片化的光谱表现出最低的可变性,主要是由于完整的质子分子峰值.
  • 鉴定出的变异源包括质量校准剂的转移,样品的不良引入和质量谱仪的污染入口.
  • 统一的方法参数显著提高了实验室间的可重复性,特别是更高的来源碰撞诱导分离.

结论:

  • AI-MS是一个强大的技术,用于查获的药物选,具有固有的高可重复性.
  • 标准化方法可以进一步优化AI-MS性能和数据的一致性.
  • 这些发现支持AI-MS在法医应用中的文档标准和错误率的发展.