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质量测量AHL-乳酶 Est816抑制多微生物下斑块衍生生物膜的形成

Zelda Ziyi Zhao¹, Wenwen Shan², Xiaoyu Sun²

¹Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China.

Dentistry journal

|August 27, 2025

概括

关闭定数的酶N-acyl-homoserine lactone (AHL) - lactonase Est816有效地抑制了下斑块生物膜的形成. 这种酶减少了微生物多样性和生物量,显示出治疗牙周炎的潜力.

科学领域:

微生物学
酵素学
牙周病学

背景情况:

牙周炎是一种复杂的口腔疾病,以多微生物生物膜的形成为特征.
牙下斑块生物膜对于晚期牙周炎的产生至关重要.
针对细菌中的定数感应是一种有前途的生物膜控制策略.

研究的目的:

评估定数灭酶N-acyl-homoserine乳 (AHL) -乳酶Est816的疗效.
研究Est816对牙周炎患者的牙下斑块微生物群的生物膜形成的影响.
评估 Est816 处理后生物膜组成,多样性和结构的变化.

主要方法:

从患有晚期牙周炎的患者中采集了牙下斑块样本.
在体外无氧培养物中使用Est816或酸盐缓冲盐水 (PBS) 作为对照剂.
生物膜分析包括16S rRNA测序,SEM,水晶紫色和CLSM.

主要成果:

显著降低了微生物丰富度 (Chao1指数,p=0. 031).
生物膜的生物量和厚度分别减少了64% (p < 0. 001) 和76% (p < 0. 001).
扫描电子显微镜在 Est816 处理的样本中发现了碎片化生物膜结构.

结论:

能有效抑制多微生物下斑块生物膜的形成.
治疗Est816导致物种丰富性,遗传多样性和社区均性减少.
Est816在牙周炎中具有破坏病原生物膜的辅助治疗潜力.

关键词:

N-乙-同氨酸乳生物片口腔微生物组检测人数的方法毒性因子

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