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Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

空间解析的植物代谢学

Ronald J Myers¹, Zachary M Tretter¹, Abigail G Daffron¹

¹Division of Biochemistry, University of Missouri-Columbia, Columbia, MO 65211, USA.

|August 27, 2025

概括

质谱成像 (MSI) 通过空间分辨代谢物积累来推进植物代谢学. 这项技术为植物生物学,耐压力和提高作物产量提供了新的洞察力.

关键词:

吸附电喷气电离质谱成像质谱学用矩阵辅助的激光脱离电离植物新陈代谢植物压力空间代谢学

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Last Updated: May 4, 2026

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

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Published on: March 13, 2020

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Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI

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Published on: December 16, 2022

科学领域:

植物生物学
代谢学
分析化学

背景情况:

传统的代谢学方法缺乏植物组织内的代谢物的空间分辨率.
质谱成像 (MSI) 已成为检测和绘制代谢物积累的强大工具.
自2010年以来,植物科学领域的MSI采用率升了350%.

研究的目的:

审查植物代谢学MSI的最新进展.
突出样本准备,MSI技术和数据处理方面的改进.
总结MSI在植物生物学中的当前应用和未来方向.

主要方法:

使用MALDI和DESI等脱离电离技术.
开发用于组织段的先进样本制备技术.
实施新的数据处理工具以克服MSI的局限性.

主要成果:

MSI提供空间解析的代谢物数据,为生物化学途径提供独特的洞察力.
最近的技术进步提高了MSI在植物研究中的准确性和范围.
越来越多的文献表明MSI的影响越来越大.

结论:

通过对代谢物进行空间分析, MSI 正在彻底改变植物代谢学.
在MSI的持续进步将推动植物耐力,发育和作物产量方面的重大发现.
未来的研究应该利用MSI进一步探索植物生化过程和农业应用.