通过增强效应体表达和抑制宿主免疫反应,对Verticillium dahliae的毒性进行负面调节
Di Xu1, Xiaoqiang Zhao1, Can Xu2
1Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, College of Agriculture, Shihezi University, Shihezi 832000, China.
Journal of fungi (Basel, Switzerland)
|August 27, 2025
概括
在Verticillium dahliae中,sarcosine oxidase基因VdSOX1通过增加效应基因表达和抑制棉花来促进真菌毒性.
科学领域:
- 植物病理学
- 分子菌学
- 农业科学
背景情况:
- 这种疾病对棉花等作物造成破坏性.
- 了解真菌致病机制对于有效的疾病管理至关重要.
研究的目的:
- 研究VdSOX1基因在V. dahliae病毒性和宿主病原体相互作用中的作用.
- 确定VdSOX1作为控制棉花血管化的潜在目标.
主要方法:
- 在V. dahliae中删除VdSOX1的基因.
- 淘汰菌株的表型分析 (病毒性,微硬质菌的形成,碳利用).
- 转录组分析以评估效应基因表达和宿主防御反应.
主要成果:
- 删除VdSOX1增加了真菌的毒性,微硬质细胞的形成和碳来源的利用.
- 预测VdSOX1将超过50个被分泌的效应基因调节.
- 抑制了VDSOX1的棉花防御转录因子 (WRKY,MYB,AP2/ERF,GRAS).
- 转录组学发现VdSOX1将营养获取与免疫逃避联系在一起.
结论:
- VdSOX1是促进V. dahliae毒性的关键调节剂.
- VdSOX1通过提高效应基因和抑制宿主免疫力来增强致病性.
- VdSOX1 是一种潜在的新目标,用于控制棉花中的Verticillium wilt.
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