协同表达网络分析表明PacC转录因子涉及Botryosphaeria dothidea在中国海中的致病性
Dong Liang1, Yiru Jiang1, Wei Ai1
1College of Advanced Agricultural Sciences, Zhejiang Agriculture and Forest University, Hangzhou 311300, China.
Journal of fungi (Basel, Switzerland)
|August 27, 2025
概括
这种植物会引起中国树干癌症. 这项研究揭示了PacC转录因子 (BDLA_00001555) 对真菌至关重要.
科学领域:
- 植物病理学
- 分子生物学
- 菌群学
背景情况:
- Botryosphaeria dothidea会导致中国树干癌症,这对Carya cathayensis的生产构成重大威胁.
- 细胞内生病原性阶段过渡或潜伏性感染是B. dothidea与宿主相互作用的关键,但仍不明确.
研究的目的:
- 调查B.dothidea在与中国红杉相互作用时的相变过程中的转录变化.
- 确定隐藏感染阶段的关键基因和调控机制.
主要方法:
- 用RNA测序 (RNA-Seq) 来分析基因表达.
- 同表达网络分析确定了差异表达基因 (DEG) 和枢纽基因.
- 进行了基因本体 (GO) 丰富分析和基因淘汰实验.
主要成果:
- 确定了与模拟潜伏感染状况相关的同表达模块.
- 检测到53个枢纽基因,在膜传输,离子稳态和碳水化合物代谢中进行丰富.
- 在病原性激活过程中,PacC转录因子 (BDLA_00001555) 和内分β- 1, 3- 葡萄糖酶 (BDLA_00010249) 的上调.
- 消除BDLA_00001555的病原性状态激活.
结论:
- 在B. dothidea的潜感染阶段,PacC转录因子BDLA_00001555起着至关重要的作用.
- 这些发现有助于了解中国树干癌的致病机制.
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