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基于基因组混杂技术的高多糖生成的Volvariella volvacea菌株的繁殖

Lihui Liang^1,2,3, Qihang Su^1,3,4, Yawei Wang^1,3,4

¹National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China.

Journal of fungi (Basel, Switzerland)

|August 27, 2025

概括

大气和室温等离子体突变和基因组混合显著改善了Volvariella volvacea中的外聚糖 (EPS) 生产. 这种新的方法开发出高产的真菌菌株,提高了多糖的产量.

科学领域:

菌群学
生物技术
生物化学

背景情况:

由于菌株的遗传异质性影响了产量和成分,Volvariella volvacea是一种为其多糖种植的真菌面临挑战.
*目前的培养方法导致多糖含量和变异性不一致.
大气和室温等离子体 (ARTP) 技术为生物突变诱导提供了一种新的方法.

研究的目的:

* 为了开发高产的外聚糖 (EPS) 菌株的Volvariella volvacea.
通过整合ARTP突变和基因组混合来克服培养瓶.
* 确定对增强EPS产生的代谢和遗传途径.

主要方法:

* 使用ARTP突变生成稳定的V. volvacea突变.
* 在精选的突变体上进行了基因组混合,涉及三轮原生质融合.
* 进行了代谢学和转录学分析以调查潜在的机制.

主要成果:

* ARTP突变产生了9种稳定突变,EPS产量超过20%.
通过基因组混合开发的混合品种SL212实现了EPS产量增加111.67% (46.85g/L).
* 在银河糖代谢,ABC转运途径和三碳酸循环中富含了差异化代谢物和基因.

结论:

* 综合ARTP突变和基因组混合是增强V. volvaceaEPS产生的有效策略.
通过确定的途径增强单糖生物合成和ATP生成有助于EPS的过度产生.
* 这项研究提供了推动真菌高聚糖生物合成的关键因素.

关键词:

一个ARTP 虫虫细胞外多糖基因组混合高多糖的产量

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