使用膜带进行增强的流过
Zachary Badinger1, Ali Behboudi1, Andrew L Zydney1
1Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
Membranes
|August 27, 2025
概括
使用单通触流过 (SPTFF) 的膜盒可连续净化沉的单克隆抗体 (mAbs). 这种强化工艺可以实现高转化率和流量,而不会造成污染,从而促进生物处理.
科学领域:
- 生物技术
- 生物处理工程
- 蛋白质净化
背景情况:
- 细胞培养的进步增加了单克隆抗体 (mAb) 标位,需要有效的下游处理.
- 持续的净化提供了加强生物制造的机会.
- 选择性沉后的过是一个潜在的连续下游过程.
研究的目的:
- 评估使用微膜的单通触流过 (SPTFF) 连续净化沉蛋白质的有效性.
- 为了比较SPTFF在空心纤维和膜磁带模块中的性能.
- 开发单克隆抗体的连续净化系统.
主要方法:
- 使用ZnCl2和聚乙烯糖醇对人体血清免疫球蛋白G进行沉.
- 在空洞纤维和选磁带模块中使用单通触流过 (SPTFF).
- 使用流量分步实验来确定临界流量.
主要成果:
- 屏蔽带达到高达250L/m2/h的关键流量,远高于空心纤维模块.
- 一个双阶段的SPTFF系统在一次通过中显示了高达85%的转换.
- 在24小时以144L/m2/h的液流量连续运行显示80%的转换没有显著的污染.
结论:
- 选的膜磁带显示了像mAbs这样的沉蛋白质的强化连续处理的巨大潜力.
- 在选的磁带中,SPTFF提供了一种高效和连续的下游净化方法.
- 这种方法促进了完全连续的生物处理工作流程的发展.
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