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Updated: Jun 15, 2026

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

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基于SBEM的培养人类细胞超结构分析的优化协议

Natalia Diak1, Łukasz Chajec2, Agnieszka Fus-Kujawa1

  • 1Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medykow 18 Street, 40-752 Katowice, Poland.

Methods and protocols
|August 27, 2025
PubMed
概括

我们为人类细胞优化了串行块面扫描电子显微镜 (SBEM). 这项新协议增强了干细胞和纤维细胞的超结构分析,提供了更好的清晰度和安全性.

关键词:
关于SBEM的协议纤维细胞其他国家细胞的超结构

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相关概念视频

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.