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DKK3和WIF1在前列腺癌中的潜在作用:生物信息学和临床分析

Zhiliang Xia¹, Zhonggui Hu¹, Dan Du¹

¹Department of Urology, Second People's Hospital of China Three Gorges University, 21 Xiling One Road, Yichang, 443000, Hubei, China.

Discover oncology

|August 27, 2025

概括

这项研究确定WIF1和DKK3是前列腺癌下调的关键基因. 它们的减少表达会影响瘤的微环境,并可能导致抗药性,这表明有可能进行精确治疗.

科学领域:

癌症学
生物信息学
分子生物学

背景情况:

前列腺癌 (PCa) 进展为抵抗割的PCa (CRPC) 存在治疗挑战.
瘤微环境 (TME) 和免疫透对于PCA的发展和治疗反应至关重要.

研究的目的:

在PCa开发中使用多omics数据集成识别核心基因.
研究核心基因在免疫微环境中的作用及其对PCa的治疗影响.

主要方法:

来自GEO和TCGA数据库的综合基因表达数据.
应用生物信息学工具,包括差异基因表达分析,PPI网络构建和机器学习 (Lasso,RF).
通过外部数据集,免疫细胞透分析,途径丰富 (GSEA),药物敏感性分析 (CTRP) 和实验证实 (IHC,Western blot) 的验证结果.

主要成果:

确定了339个差异表达基因 (DEG) 和四个核心基因 (DKK3,SNAI2,WIF1,FOXA1).
在PCa中,DKK3和WIF1显著下调,与更高的PSA和格里森分数相关.
DKK3和WIF1的下调与免疫力受损,矩阵重塑和Wnt/TGF-β通路失调有关.

结论:

WIF1和DKK3是PCa显著下调的瘤抑制剂.
通过影响Wnt/TGF-β通路和免疫微环境,它们的失活促进了PCa的进展和耐药性.
恢复WIF1和DKK3提供了早期诊断和精确治疗PCa的潜在策略.

关键词:

生物信息学丹麦克朗免疫透机器学习前列腺癌 WIF1 时间

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