来自Thinopyrum junceum的树干抗基因Sr68进入小麦
Swarupa Nanda Mandal1,2, Baljeet K Gill3, Zhixia Niu4
1Department of Plant Sciences, University of California, Davis, CA, 95616, USA.
概括
一个新的干抗基因,Sr68,已成功从Thinopyrum转移到小麦染色体1BS. 这为小麦提供了对Puccinia graminis f. sp的抗药性. 可能会降低质免疫性.
科学领域:
- 植物遗传学和育种
- 农作物保护
- 分子生物学
背景情况:
- 茎生,由葡萄菌引起 对于全球小麦产量来说,
- 识别和部署新型干抗基因对于可持续的小麦种植至关重要.
- 这种Thinopyrum junceum物种是小麦改良的有价值的外来遗传物质来源.
研究的目的:
- 在小麦中识别和侵入Thinopyrum junceum的新型干抗基因.
- 描述进化基因的位置和遗传性质.
- 评估新的小麦品种对疾病耐药性和减少质免疫性的潜力.
主要方法:
- 在小麦-Thinopyrum连接线 (HD3505) 和小麦单体4D染色体之间的交叉中开发F2种群.
- 使用单体诱导的罗伯逊转位和ph1b诱导的同质重组进行基因转移.
- 使用基因组和寡核酸多重光 in situ 杂交 (FISH) 进行精确的染色体定位.
主要成果:
- 一个名为Sr68的新干抗基因从Th成功转移. 在小麦染色体臂1BS的距离区域.
- 三条小麦线 (BG2133,BG5136,BG2161) 含有Th. 通过Sr68开发出结合性染色体.
- 内进取代了Gli- B1位点,导致 ω- gliadins的消除,可能降低了质免疫性.
结论:
- 新的Sr68基因提供了对Ug99和其他Pgt种族的新抗药源.
- 使用Sr68开发的小麦品种为旨在增强小麦对茎的抗性提供了宝贵的遗传资源.
- 消除 ω-gliadins 提供了开发潜在降低过敏性的小麦品种的机会.
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