转录因子TrStuA 调节植物生长,病毒性和菌素合成
Manli Sun1, Zihan Meng1, Yichen Yang1
1State Key Laboratory of North China Crop Improvement and Regulation/College of Plant Protection, Hebei Agricultural University, Baoding, Hebei 071000, China.
转录因子StuA对于收获后果病原体Trichothecium roseum产生的三毒素 (TCN) 是必不可少的. 删除StuA显著降低了TCN水平,生长和致病性,提供了一个新的真菌杀菌剂目标.
科学领域:
- 菌群学
- 植物病理学
- 分子生物学
背景情况:
- 虫 (Trichothecium roseum) 导致果收获后腐烂,并产生菌毒素菌素 (TCN),这对人类健康造成了担忧.
- 在T. roseum中TCN合成的调节还不清楚.
研究的目的:
- 研究T. roseum中的APSES型转录因子Stua的功能.
- 确定StuA在TCN生物合成,真菌生长和致病性中的作用.
主要方法:
- 基因淘汰和补充以创建一个 ΔTrStuA 突变.
- 转录组分析和qRT-PCR以评估基因表达.
- 高性能液体染色学 (HPLC) 用于量化TCN水平.
主要成果:
- 这种 ΔTrStuA 突变显示了植物生长,结合,应激耐受性和致病性降低.
- TrStuA积极调节TCN生物合成,TCN在ΔTrStuA突变体中无法检测到.
- TrStuA影响了二次代谢基因的表达,包括TRI群.
结论:
- TrStuA是T. roseum中TCN生物合成的关键积极调节剂.
- 在T. roseum的生长,发育和毒性中,StuA起着至关重要的作用.
- TrStuA是开发杀菌剂控制TCN污染和粉红色腐烂的潜在目标.
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