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离心力和时间对狗血小板,白细胞和生长因子度的影响

Mariana Fernandez¹, Nina R Kieves¹

¹Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH.

Journal of the American Veterinary Medical Association

|August 27, 2025

概括

使用ACP Max系统优化对狗血小板丰富血的离心,可提高血小板和生长因子度. 对血小板和PDGF-ββ水平产生了最好的结果.

科学领域:

动物医学
复原医学
生物技术

背景情况:

富于血小板的血 (PRP) 用于其再生特性.
优化PRP制备对于最大化治疗潜力至关重要.
狗PRP研究对于兽医再生疗法的发展至关重要.

研究的目的:

评估不同的离心力和旋转时间如何影响狗PRP的组成.
确定用于犬类PRP的自生条件等离子体 (ACP) 最大系统的最佳设置.
分析对血小板,白细胞,红细胞和生长因子度的影响.

主要方法:

使用四种初始旋转方案 (2,420或2,696克6或9分钟) 来处理健康狗的全血.
所有样品都采用了标准化的第二次旋转周期.
使用全血细胞计和ELISA测量细胞成分和生长因子 (PDGF-β,FGF,TGF-β1,IGF-1,VEGF,IL- 1RA).

主要成果:

与全血相比,所有方案都减少了红细胞和白细胞,同时增加了血小板.
一个2,420 x g的初始旋转显著增加了血小板度.
在9分钟的2420克方案显示了血小板和PDGF-β度的显著增加.

结论:

通过ACP Max系统有效地产生犬类PRP,并产生所需的细胞变化.
在提高血小板和PDGF-ββ水平方面, 9 分钟的2 420 x g离心方案是最有效的.
需要进一步的研究来将这些PRP变异与临床结果相关联.

关键词:

时间周期白细胞富含血小板的血血小板旋转周期

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