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Sorbhi Rathore¹, Julian Conrad², Dasmanthie De Silva³

¹Department of Biochemistry and Biophysics, Stockholm University. Stockholm SE-10691, Sweden.

Biochimica et biophysica acta. Molecular cell research

|August 27, 2025

概括

在Saccharomyces cerevisiae中,需要组装因子Mrh4,Mtg1和Mtg2来进行线粒体大子单元 (mtLSU) 生物发生. 这些因素在晚期mtLSU成熟过程中协调rRNA折叠和蛋白质结合.

科学领域:

分子生物学
细胞生物学
生物化学

背景情况:

在Saccharomyces cerevisiae中, mitochondrial DNA编码的蛋白质被合成为氧化酸化.
线粒体大子单元 (mtLSU) 生物发生是一个复杂的过程,涉及许多组装因素.

研究的目的:

在晚期mtLSU组装过程中阐明DEAD盒螺旋酶Mrh4和GTPasesMtg1/GTPBP7和Mtg2/GTPBP5的等级作用.
了解这些因素在rRNA折叠和线粒体核糖体蛋白 (MRP) 结构中的作用.

主要方法:

基因分析
生物化学试验
在体外溶解
冷电子显微镜 (冷电子显微镜)

主要成果:

通过 Mrh4 介导的 bL33m 结合会在 Mtg1 加入 21S rRNA 之前.
Mtg1重组了关键的21SrRNA域 (H73-75,H93),使其能够进行后续的螺旋结构和MRP整合 (uL6m,uL16m,bL35m,bL36m).
含有mtLSU的不成熟单体聚集在耗尽的线粒体中,这表明这些因素在防止异常聚集方面发挥了作用.

结论:

这项研究揭示了Mrh4,Mtg1和Mtg2在晚期mtLSU成熟中的顺序作用.
它突出了线粒体核糖体组合的保存机制,特别是rRNA折叠和MRP结构.
这一发现为复杂的线粒体生物发生过程提供了洞察力.

关键词:

化器死亡盒螺旋酶一个GTPase 线粒体核糖体组合第四章其他:氧化化酵母 bL33m 其他其他国家

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