使用大米来制备培养基,以合成乳酸细菌生物膜和调节料发酵
Zhumei Du1, Jiachen Fang2, Huili Pang3
1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Bioresource technology
|August 27, 2025
概括
这项研究创建了一个低成本的米介质,用于生产乳酸细菌 (LAB) 生物膜,用于优质料发酵. 开发的LAB生物膜改善了料质量,并促进了有益的微生物转移.
科学领域:
- 农业科学
- 微生物学
- 生物技术
背景情况:
- 草等作物副产品是未充分利用的资源.
- 料发酵质量对于动物料的保存至关重要.
- 有害的微生物可能会占主导地位,损害料的质量.
研究的目的:
- 开发一种低成本的天然培养基,使用大米来生产乳酸细菌 (LAB) 生物膜.
- 研究LAB生物膜在改善料发酵质量的有效性.
- 分析LAB生物膜在化过程中对微生物群体结构的影响.
主要方法:
- 草被用来创造一个自然的培养基.
- 使用的是乳酸细菌 (LAB) 菌株FG1 (Lactiplantibacillus plantarum) 和TH14 (Lacticaseibacillus casei).
- 使用开发的介质和LAB菌株从果园草和蒂莫西草中制备料.
- 测量了发酵质量参数 (pH,氨,乳酸).
- 分析了微生物群落结构和代谢途径.
主要成果:
- 使用FG1介质发酵的料显示出优良的质量 (pH<3.6,氨<0.7g/kg FW,乳酸>1.1g/kg FW).
- 这种 LAB 生物膜促进了由 Lactiplantibacillus plantarum (35. 4%) 占主导地位的从格兰阴性细菌转变为格兰阳性细菌.
- 生物膜调节了微生物共生网络,增强了碳水化合物代谢并抑制了氨基酸通路.
结论:
- 一种低成本的米草基媒介有效地产生了LAB生物膜,用于高质量的料.
- 设计的LAB生物膜结构显著改善了料发酵和微生物动力学.
- 这种方法提供了一种可持续且有效的低成本,高质量的料生产方法.
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