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使用工程Issatchenkia orientalis从酒的花中生产3-基酸

Linjing Jia¹, Deokyeol Jeong², Mairui Zhang¹

¹Carl and Melinda Helwig Department of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506, USA.

Journal of biotechnology

|August 27, 2025

概括

这项研究表明,使用新型的CaO预处理和工程酵母,从酒厂的消耗谷物中生产3-基酸 (3-HP). 脱蛋白化BSG产生了最高的3HP标位,显示出高效的生物质利用.

科学领域:

生物技术
生物化学工程
工业微生物学

背景情况:

3-基酸 (3-HP) 是一种具有多种工业应用的关键平台化学物质.
酒用谷物 (BSG) 是造过程中丰富且低成本的纤维素副产品,是可持续的原料.
有效地将BSG转化为3-HP等有价值的化学物质需要优化预处理和发酵策略.

研究的目的:

开发一种概念验证,使用单CaO预处理和一种耐酸酵母菌株从BSG中生产3-HP.
研究包括酸类型,源,NaHCO3添加和BSG脱蛋白化在内的各种因素对3-HP生产的影响.
建立一个强大的发酵工艺,从生物质中生产具有附加价值的化学物质,而无需外部补充.

主要方法:

在BSG水解中采用了一种新的单CaO预处理方法.
在3-HP发酵中使用了耐酸的Issatchenkia orientalis IoDY01H菌株.
优化实验评估了不同酸类型 (H2SO4,H3PO4) 的pH调整,源 (酵母提取物与合成完整介质),NaHCO3补充和BSG脱蛋白.

主要成果:

与使用纯糖的合成完整介质相比,酵母提取物介质增强了3-HP标位13.3%.
在YPDX介质中,NaHCO3补充增加了3-HP标位至7. 8g/ l.
在BSG水解剂中调整pH的H2SO4产生的3-HP (7.4g/L) 比H3PO4 (6.8g/L) 高.
在没有补充的情况下,BSG的脱蛋白化显著改善了的回收,并导致最高的3-HP标位为8.7g/L.

结论:

该研究成功地证明了使用一预处理和发酵方法从BSG中生产3-HP.
优化预处理条件,特别是脱蛋白化和发酵参数,如pH调整和NaHCO3添加,对于最大限度地提高3HP产量至关重要.
开发的过程提供了一种可持续的途径,使BSG成为有价值的化学品,突出了无发酵的潜力.

关键词:

3基酸酒厂的花费谷物脱蛋白化东方鱼

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