富含RubisCO的可食海叶蛋白缩物的构成和发泡特性
Wenjuan Feng1, Zhihao Huang1, Bo Pan2
1State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China.
International journal of biological macromolecules
|August 27, 2025
概括
富含RuBisCO的可食用海叶蛋白缩物 (RPI) 具有卓越的适应pH的发泡特性. 这种可持续的植物性蛋白质的性能与动物蛋白质相美, 非常适合食品应用.
科学领域:
- 食品科学
- 蛋白质化学
- 可持续的原料
背景情况:
- 鲁比斯科是最丰富的植物蛋白质,为动物蛋白质提供可持续的替代品.
- 可食用叶蛋白缩剂 (RPI) 是一种使用过法提取的新型蛋白质来源.
- 了解RPI的pH诱导的形状变化对于其作为功能成分的应用至关重要.
研究的目的:
- 将RPI与商业蛋白质分离物的pH诱导的形状变化和发泡特性进行比较.
- 为量身定制的食品应用阐明RPI的结构功能关系.
- 确定RPI作为一种适应pH的植物性发泡剂.
主要方法:
- 用于RPI提取的超.
- 动态光散射以测量泽塔潜力和粒子大小.
- 用于测定表面疏水性和微环境的ANS结合测试和内在光.
- 测量度以评估聚合.
- 在pH范围内进行泡容量和稳定性测试 (5-9).
主要成果:
- 与其他蛋白质相比,RPI在pH调节下表现出更大的灵活性.
- 在pH > pI时,RPI显示负泽塔电位增加 (- 8. 63mV至 -23. 65mV) 和表面疏水性降低 (-47. 36%).
- 在pH值5 - 9的情况下,RPI表现出优异的泡容量 (110 - 140%) 和稳定性 (> 85%),与动物蛋白质相美.
结论:
- RPI具有独特的界面分子排列,具有pH诱导的灵活性和平衡的静电/疏水相互作用.
- 这些特性可以形成机械稳定的薄膜和持续的泡结构.
- RPI是一种高度适应性的植物性发泡剂,适用于各种食品应用.
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