相关实验视频

Updated: Nov 9, 2025

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Published on: October 25, 2024

Malat1通过控制早期分化和对IL-2的反应来调节女性Th2细胞的细胞因子表达

Mags Gwynne^1,2, Katie A West^1,2,3, Stijn van Dongen⁴

¹Hull York Medical School, University of York, York, United Kingdom.

Journal of immunology (Baltimore, Md. : 1950)

|August 27, 2025

概括

长非编码RNA Malat1对于女性T辅助细胞的适当细胞因子产生至关重要,它影响免疫反应,并揭示了免疫性二态的关键因素.

科学领域:

免疫学
分子生物学
遗传学

背景情况:

免疫系统反应表现出显著的性二态,影响各种免疫病态的易感性和严重性.
了解免疫性二态的细胞内在调节者对于开发向疗法至关重要.

研究的目的:

研究长非编码RNA Malat1在调节T助手2 (Th2) 细胞分化和功能的作用,特别是关于性别差异.
阐明马拉特1对雌性与雄性小鼠Th2细胞反应的影响的分子机制.

主要方法:

在实验室中从马拉特1缺陷 (Malat1-/-) 和野生型 (WT) 小鼠中分化原始的CD4+T细胞.
全转录组分析 (RNA测序) 来评估基因表达的变化.
细胞因子表达概况和受体阻断实验 (IL-10R).
用干扰素β治疗以模仿马拉特1损失的效果.
在2型炎症中使用Schistosoma mansoni卵模型的体内研究.

主要成果:

在雌性小鼠中,马拉特1缺乏会影响Th2分化,抑制IL-10,IL-4和IL-13等关键细胞因子.
在机理上,女性的 Malat1 损失导致早期激活的改变,差异化基因表达的受损,以及IL-2 受体信号的减少.
虽然在男性细胞中观察到一些影响,但终点Th2分化没有受到影响,这突显了性别特异性调节.
在体内,雌性马拉特1-/-小鼠在 Schistosoma mansoni 卵刺激后,肺部和脏中的 IL- 10+ Th2 细胞减少.

结论:

马拉特1是Th2细胞分化和细胞因子生产的关键细胞内在调节剂,特别是在女性中.
这些发现揭示了马拉特1是免疫性二态的新型决定因素.
这些见解可以为性别偏差免疫病理的治疗策略提供信息.

关键词:

马拉特人一个IL-10 Th2 细胞免疫性变态链接RNAs

更多相关视频

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

相关概念视频

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...