在体外生产的牛早期胚胎中的性别偏差转录组
Meihong Shi1, Guangsheng Li1, Hannah Marie Araujo1
1Department of Animal Science, College of Agriculture and Life Science, Cornell University, Ithaca, NY, USA.
Cell & bioscience
|August 27, 2025
概括
由于丰富的新陈代谢,雄性牛胚胎的发育速度更快,通过分析胚芽细胞的基因表达和替代拼接差异来揭示. 这项研究揭示了推动早期胚胎性别差异的关键分子机制.
科学领域:
- 生殖生物学
- 发育生物学
- 基因组学
背景情况:
- 胚胎的性别是由染色体在受精时决定的,
- 男性胚胎,尤其是试管婴儿胚胎,往往比女性胚胎发育得更快.
- 这些早期胚胎性别差异的分子基础在很大程度上是未知的.
研究的目的:
- 调查早期牛胚胎发育中的转录性差异.
- 确定推动性别特异性发育速度的分子机制.
- 分析胚胎细胞中性别偏差基因表达,替代拼接和异形动力学.
主要方法:
- 采用PCR检测采集了体外产生的牛胚囊,并对其进行了性别测定.
- 进行超低输入RNA测序 (RNA-seq) 来分析转录组.
- 鉴定和分析了差异性基因表达 (DEG),差异性替代拼接 (AS) 和差异性表达的异构体 (DEI).
主要成果:
- 雄性胚胎细胞的发育显著加快,并增强了新陈代谢调节途径.
- 女性胚胎囊表现出与性腺发育,性别分化和TGF-β信号传递相关的途径.
- 发现了性别偏差的替代拼接和异形表达,影响了代谢和转录调节.
结论:
- 转录组分析揭示了牛胚囊中显著的性别偏差基因表达,替代拼接和异形动力学.
- 男子胚胎的新陈代谢过程可能有助于它们的加速发育.
- 这项研究提供了有关早期胚胎形成的性别特异性调节机制的关键见解.
相关概念视频
Crossing Over
Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
The Ratio of X Chromosome to Autosomes
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Dosage Compensation
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...


