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Updated: Sep 10, 2025

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening
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在癌症中对微RNA生物标记物的无放大测试

Bahareh Soleimanpour1,2,3,4,5, Juan Jose Diaz Mochon2,3,4,5, Salvatore Pernagallo1

  • 1Destina Genomica S.L., Parque Tecnológico de la Salud de Granada (PTS), Avenida de la Innovación 1, Edificio BIC, 18016 Granada, Spain.

Cancers
|August 28, 2025
PubMed
概括

开发无放大技术对于可靠的癌症诊断至关重要. 这些无PCR方法克服了传统PCR的局限性,使得精确医学能够准确检测循环中的microRNA.

关键词:
没有PCR的技术没有放大功率的平台生物标志物分析癌症诊断早期检测没有标签的检测液体活检微RNAs (miRNAs) 的使用多重测试核酸检测基于传感器的检测

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MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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