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全面纵向线性混合模拟CTC揭示了Trop2,EpCAM和CD45在CTC聚类和转移中的作用

Seth D Merkley¹, Huining Kang², Ursa Brown-Glaberman³

¹Division of Molecular Medicine, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA.

|August 28, 2025

概括

循环瘤细胞 (CTC) 的异质性会影响乳腺癌的进展. EpCAM,Trop2和CD45+细胞预测CTC群的大小,随着时间的推移观察到HR+和HER2+转移性乳腺癌的显著生物标志物变化.

科学领域:

癌症学
癌症生物学
转移研究

背景情况:

乳腺癌是全世界最常见的癌症,
循环瘤细胞 (CTC) 是转移的关键驱动因素,与预后不佳有关.
CTC异质性使理解它们在疾病进展中的作用变得复杂.

研究的目的:

系统地对转移性乳腺癌患者的循环瘤细胞 (CTC) 进行纵向分析.
评估 EpCAM,Trop2 和泛细胞素 (CK) 标记物对 CTC 存在和聚类的贡献.
了解CTC生物标志物与乳腺癌在治疗过程中的进展之间的关系.

主要方法:

对51名转移性乳腺癌患者的272个血液样本进行了纵向分析.
使用Rarecyte®技术分离,成像和分析核细胞.
使用R (lmerTest, lme4) 和Graphpad Prism进行统计分析.

主要成果:

检测到古典CTC和Trop2+CTC;EpCAM,Trop2和CD45+细胞与CTC群大小相关.
泛细胞激素 (CK) 没有预测集群大小.
纵向分析显示了EpCAM,Trop2和HR+与HER2+乳腺癌转移后诊断中的不同生物标志物轨迹.

结论:

EpCAM+,Trop2+和CD45+细胞可以预测CTC的存在和大小.
在HR+和HER2+转移性乳腺癌之间观察到明显的纵向生物标志物变化.
这些发现提高了对乳腺癌进展的CTC动态的理解.

关键词:

CD45 其他一个CTC CTC 集群细胞素美国其他乳腺癌转移液体活检

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