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Kirsten E Scoggin¹, Fatma Adlan², Carleigh E Fedorka³

¹Department of Veterinary Science, University of Kentucky, Lexington, KY 40546, USA.

|August 28, 2025

概括

胰岛素类生长因子 (IGF) 系统,特别是IGF1,对马胎盘发育和血管生成至关重要,在妊娠期间与血管内皮生长因子 (VEGF) 表达呈正相关性.

科学领域:

生殖生物学
内分泌学
马类科学

背景情况:

胰岛素类生长因子 (IGF) 系统对哺乳动物的怀孕至关重要,它影响胎盘发育和血管生成.
关于IGF系统的作用及其与马胎盘 (chorioallantois; CA) 和孕期子宫内膜 (EN) 中血管内皮生长因子 (VEGF) 的关系的信息有限.

研究的目的:

在整个妊娠期内研究马类CA和EN中的IGF系统组件 (连接体,受体,结合蛋白) 的表达模式.
检查IGF系统表达,特别是IGF1和IGF2,与马CA中的VEGF表达之间的相关性.
在马孕期确定IGF1的局部和血清度.

主要方法:

在不同妊娠年龄 (45天至11个月) 和分娩后的马体中,对IGF配体 (IGF1,IGF2),受体 (IGF1R,IGF2R,INSR) 和结合蛋白 (IGFBPs,IGF2BPs) 的mRNA表达量的定量分析.
在马体内定位IGF1的免疫组织化学.
在妊娠期间测量血清IGF1度.

主要成果:

马CA中的IGF1mRNA表达在妊娠6个月达到峰值,与VEGF表达呈正相关性 (r=0. 62,p=0. 001).
在整个妊娠期内,IGF2mRNA在马CA中比IGF1更为丰富,但其表达与VEGF没有相关性.
在孕期早期 (45日) 的IGFBP2和IGFBP2的表达很高,而在产后的IGFBP3和IGFBP5的表达很高. 在9个月的妊娠期,IGF1定位在胆膜上,而血清IGF1达到峰值.

结论:

马的IGF系统,特别是IGF1,在胎盘发育和血管生成中起着重要作用.
在孕期的马体内,IGF1与VEGF表达之间存在强烈的正相关性,这表明IGF1调节了VEGF介导的血管生成.
IGF结合蛋白的差异表达表明在整个马妊娠期间具有特定阶段的作用.

关键词:

国际粮食基金系统一个 VEGF 霍里奥阿兰托斯马类胎盘

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