优化精子保存:氧气暴露,样本旋转和HEPES缓冲补充的影响
Khomsan Buathalad1, Thirawat Koedkanmark1, Wuttigrai Boonkum1,2
1Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Animals : an open access journal from MDPI
|August 28, 2025
概括
优化用于人工授精的精子保存包括使用IGGKPh扩展剂和特定的储存条件. 补充HEPES缓冲剂进一步提高精子的运动性和活力,这对于成功的家禽养殖计划至关重要.
科学领域:
- 动物科学
- 生殖生物学
- 生物化学
背景情况:
- 在家禽人工授精 (AI) 需要高质量的精子来实现最佳生育.
- 在冷藏精液的过程中, 保持精子活力和受精能力是个挑战.
- 氧化应激和代谢变化会影响储存期间的精子质量.
研究的目的:
- 研究物理和生化策略,以优化精子的保存和受精能力.
- 在不同的储存条件下评估两种精液扩展剂 (0.9%的化和IGGKPh) 的疗效.
- 在模拟AI条件下评估HEPES缓冲器和处理温度对精液质量的影响.
主要方法:
- 进行了两个因数实验,以测试精液扩展剂,氧气暴露,管旋转,HEPES缓冲剂补充和处理温度.
- 精液质量通过渐进的运动性,活力,pH值和马隆迪甲 (MDA) 度进行评估.
- 在母人工授精后确定了生育率.
主要成果:
- 在冷藏期间,IGGKPh扩展剂在保持精子运动性和活力方面显著优于NaCl.
- 有氧条件和管旋转改善了运动性,但增加了氧化应激 (MDA水平).
- HEPES缓冲剂补充剂降低了MDA水平并改善了精子质量,在冷却处理时观察到最高的生育率.
结论:
- 对AI的最佳精液保存包括代谢支持 (IGGKPh,HEPES),充足的氧气,温和的混合和pH稳定.
- IGGKPh扩展剂,特别是当补充HEPES缓冲剂并在适当条件下储存时,显著提高了的精液质量和生育能力.
- 这些发现为改善家禽人工智能的成功提供了实际指导方针,特别是在具有变化条件的野外环境中.
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