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新兴多药耐药OXA-48产生ST135的基因组洞察

Angeliki Mavroidi¹, Elisavet Froukala², Nick Spanakis²

¹Department of Microbiology, General University Hospital of Patras, 26504 Rio, Greece.

Antibiotics (Basel, Switzerland)

|August 28, 2025

概括

这项研究报告了希腊首个产生OXA-48类碳酶的Proteus mirabilis菌株. 这种多药耐药菌株属于国际克隆ST135,强调需要对这些肠道细菌进行监测.

科学领域:

微生物学
基因组学
抗菌药物耐药性

背景情况:

产生OXA-48类碳酶的肠道细菌是全球越来越严重的健康问题.
很少报告含有OXA-48类卡巴酶的Proteus mirabilis菌株.
这些菌株的特征对于了解它们的流行病学和传播至关重要.

研究的目的:

来自希腊的一种新型OXA-48类碳酶产生的Proteus mirabilis菌株 (Pm GR-1).
与其他已知的OXA-48类碳酶生成菌株进行基因组学和比较基因组学分析.
评估已识别的菌株的遗传相关性,抗菌素耐药性和毒性因素.

主要方法:

使用Vitek®紧和质谱的表型和生化表征.
抗菌敏感性测试和β-乳糖酶检测
多部位序列类型 (MLST) 和全基因组序列 (WGS).
在移动遗传元素 (MGE),基因组岛屿 (GI),抗菌耐药性基因 (ARG) 和毒性因子 (VF) 的分析中.
遗传学,核心基因组SNP和比较基因组分析.

主要成果:

从希腊医院门诊尿样中分离的Pm GR-1菌株表现出多种药物耐药性,仅对阿米卡辛和塞夫塔齐/阿维巴克坦敏感.
它含有多个β-乳糖酶基因 (blaOXA-48,blaCTX-M-14,blaTEM-1,blaTEM-2) 和其他ARG,以及醇抗性突变.
该菌株属于国际克隆ST135,与来自德国和美国的OXA-48产生的P. mirabilis菌株具有遗传相似性,具有共同的关键遗传结构和毒性因素.

结论:

这项研究代表了在希腊首次报告具有blaOXA-48的Proteus mirabilis菌株.
在不同地区的国际克隆ST135菌株中出现和传播blaOXA-48是严重的公共卫生问题.
为了减轻这些菌株的影响,必须在One Health框架内对这些菌株进行持续监测.

关键词:

其他国家在MLST 其他:类动物机械设备碳酸比较基因组学基因组学

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