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Updated: May 2, 2026

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Published on: August 9, 2011

简化针对Listeria monocytogenes的菌株选择,使用在多层验证管道中集成的基于光的感染试验

Blanca Lorente-Torres¹, Pablo Castañera¹, Helena Á Ferrero¹

¹Departamento de Biología Molecular, Área de Microbiología, Universidad de León, 24071 León, Spain.

Antibiotics (Basel, Switzerland)

|August 28, 2025

概括

一个新的选管道确定了Bacillus subtilis CECT 8266作为一种强大的益生菌. 这种菌株通过抑制细菌复制和减少体内病原体负荷,有效地预防Listeria monocytogenes感染.

科学领域:

食品微生物学
益生菌
细菌病变

背景情况:

作为一种食源性病原体,Listeria monocytogenes对公众健康构成重大风险.
它入侵宿主细胞的能力需要控制其传播和影响的策略.

研究的目的:

开发和验证用于识别益生菌菌株的查管道.
评估这些菌株对Listeria monocytogenes的疗效.

主要方法:

对26种Bacillus分离物进行了抗菌活性,胃肠性和细胞粘附性查.
使用基于光的测试来评估对L. monocytogenes的细胞保护.
在实验室和体内使用小鼠菌病模型验证了有前途的菌株.

主要成果:

细菌CECT 8266完全抑制了细胞内L.单细胞基因的复制.
在体内研究显示,脏细菌负荷减少了6倍.
基因组分析揭示了细菌素集群,并确定了与安全相关的遗传元素.

结论:

开发的管道有效地识别了具有保护能力的益生菌菌株.
由于其已证明的有效性,弹性和安全性,Bacillus subtilis CECT 8266对食品安全和健康应用具有显著的潜力.

关键词:

细菌种类.在CFU验证李斯特菌单细胞菌抗微生物管道细菌素基于光的感染模型主体与病原体的相互作用在体内保护微生物生物控制益生菌查

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