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在3T3-L1细胞中验证家庭基因RT-qPCR数据的计算方法的比较评估

Zhenya Ivanova1, Natalia Grigorova1, Valeria Petrova1

  • 1Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria.

Biomedicines
|August 28, 2025
PubMed
概括

这项研究确定了经过后生物治疗的脂肪细胞中最稳定的管理基因以进行准确的基因表达分析. 发现HPRT是RT-qPCR正常化最可靠的内部控制方法.

关键词:
3T3-L1 其他在HPRT类动物RT-qPCR 在标准化参考基因

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