相关实验视频

Updated: Sep 10, 2025

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Published on: February 17, 2023

在牙周炎中通过MAPK途径向炎症微环境的GelMA@ginsenoside Rb3

Jinmeng Sun¹, Minmin Sun¹, Zekun Li¹

¹School of Stomatology, Shandong Second Medical University, Weifang 261053, China.

Gels (Basel, Switzerland)

|August 28, 2025

概括

一种基于GelMA的新型药物输送系统GelMA@G-Rb3,通过减少炎症和促进组织再生,有效治疗牙周炎. 这种系统调节关键信号通路, 为牙周病提供有前途的治疗方法.

科学领域:

生物材料科学
复原医学
牙周病学

背景情况:

牙周炎是一种常见的炎症性疾病,导致严重的组织破坏.
目前治疗牙周炎的疗法在疗效和再生方面存在局限.
丁胺Rb3 (G-Rb3) 具有潜在的治疗特性,但有效的治疗具有挑战性.

研究的目的:

为G-Rb3 (GelMA@G-Rb3) 开发一种基于凝甲烯基 (GelMA) 的药物输送系统.
研究GelMA@G-Rb3在体外和体内治疗牙周炎的疗效.
阐明GelMA@G-Rb3在牙周炎治疗中的潜在分子机制.

主要方法:

牙周带干细胞 (PDLSC) 的分离和特征.
使用CCK-8,RT-PCR,ELISA和西班牙血栓对PDLSC中脂聚糖 (LPS) 诱导的炎症的GelMA@G-Rb3影响的体外评估.
网络药理学分析以预测机制.
在小鼠牙周炎模型中的体内评估,使用Micro-CT,H&E,Masson和免疫光染色.

主要成果:

凝MA@G-Rb3成功释放了G-Rb3并进行了特征化.
在体外,GelMA@G-Rb3通过抑制p38/ERK和激活PI3K/AKT通路,显著降低了PDLSC中LPS诱导的炎症.
在体内,GelMA@ G- Rb3显著降低了膜骨的吸收,增强了牙周组织的再生,并调节了MAPK信号通路.

结论:

凝MA@G-Rb3是一种有效的牙周炎药物输送系统.
该系统调节炎症反应并促进牙周组织再生.
这项研究为牙周炎的创新治疗策略提供了基础.

关键词:

这是一个很大的问题.MAPK 途径人参化物Rb3 牙周带干细胞牙周炎

更多相关视频

Isolation, Processing and Analysis of Murine Gingival Cells

Isolation, Processing and Analysis of Murine Gingival Cells

Published on: July 2, 2013

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

相关概念视频

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...