在斯科波胺诱导的认知障碍斑马鱼模型中,通过益生菌配方进行神经行为保护
Emanuel Vamanu1,2, Ionela Avram3, Diana Roxana Pelinescu3
1Faculty of Biotechnology, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 011464 Bucharest, Romania.
Life (Basel, Switzerland)
|August 28, 2025
概括
两种益生菌配方有效降低了斑马鱼中斯科波胺诱导的认知功能障碍和焦虑. 配方P4也表现出抗炎作用而没有毒性,这表明神经精神疾病的治疗潜力.
科学领域:
- 神经科学
- 药理学
- 行为科学
背景情况:
- 认知功能障碍和焦虑是神经精神疾病的重大挑战.
- 斯科波胺 (SCOP) 是一种广泛使用的药物剂,用于诱导动物模型中的认知缺陷和焦虑类行为.
- 益生菌因其肠道健康益处而闻名, 越来越多地研究其对大脑功能和行为的潜在影响.
研究的目的:
- 评估两种益生菌配方 (P1和P4) 在减轻成年斑马鱼中斯科波胺诱导的认知功能障碍和焦虑相关行为的有效性.
- 在体外和体内评估益生菌配方的抗炎和细胞毒性.
- 探索益生菌在治疗神经精神疾病方面的治疗潜力.
主要方法:
- 成年斑马鱼 (Danio rerio) 用斯科波胺 (100μM) 诱导认知和焦虑缺陷.
- 行为评估包括新型坦克测试 (NTT),Y迷宫研究和新型物体识别测试 (NOR).
- 进行了体外和体内测试,以评估P1 (ColonX) 和P4 (AnXietate提取物) 的抗炎活性和细胞毒性.
主要成果:
- 这两种益生菌配方 (P1和P4) 显著改善了斑马鱼的斯科波胺诱导的行为障碍.
- 通过Y迷宫和NOR测试测量认知性能, 通过益生菌治疗得到改善.
- 益生菌配方P4表现出显著的抗炎活性,没有可观察到的细胞毒性.
结论:
- 在减轻认知缺陷和焦虑相关行为方面,
- 配方P4具有有益的抗炎特性,没有有害的细胞毒性作用.
- 这些发现表明益生菌可能是神经精神疾病的可行治疗策略.
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