在形肌肉瘤中,肌在平滑肌肉分化中的潜在参与
Hiroko Onagi1, Raku Son2, Akiko Oguchi2
1Department of Human Pathology, Juntendo University School of Medicine, Tokyo 113-8421, Japan.
International journal of molecular sciences
|August 28, 2025
概括
在高等级的肉瘤中,肌素在平滑肌肉分化中起作用. 减少肌素的表达可以作为雷肌肉瘤和形雷肌肉瘤的诊断标志物.
科学领域:
- 癌症学
- 分子生物学
- 遗传学
背景情况:
- 高度瘤通常缺乏明确的区分,使诊断复杂化.
- 形肌肉肉瘤 (PLMS) 是一种高等级的肉瘤,具有肌肉肉肉瘤 (LMS) 的特征和不分化区域.
- 了解肌细胞分化机制对于瘤诊断至关重要.
研究的目的:
- 调查基因表达网络中的肌体差异化.
- 使用基因表达的CAP分析 (CAGE) 来区分PLMS与其他高等级的肉瘤.
- 探索肌素 (MSTN) 和α光滑肌动蛋白 (ACTA2) 在肉瘤分化中的作用.
主要方法:
- 在27个高等级肉瘤样本 (10个PLMS,11个myxofibrosarcomas,3个liposarcomas,2个US,1个NOS) 上进行CAGE分析.
- 基于差异表达的基因进行分层聚类.
- 肌素和光滑肌肉的表达的免疫组织化学分析.
- 对59例软组织肉瘤 (27例PLMS,16例LMS) 的验证研究.
主要成果:
- CAGE分析区分了大多数PLMS病例与其他高等级的肉瘤.
- 在缺乏光滑肌分化的肉瘤中,MSTN促进剂的激活率更高,而在PLMS中,ACTA2促进剂的激活率更高.
- 在其他类型的瘤中,PLMS的表达减少或不存在,与扩散表达形成鲜明对比.
- 肌和肌原性标记物表现出相互排斥的表达模式.
- 在LMS和PLMS中证实了肌激素表达的损失/ 减少 (大约1%) 63%) 的情况.
结论:
- 在高等级的肉瘤中,肌素有助于平滑肌肉的分化.
- 肌素表达水平可以作为高等级瘤的潜在诊断标志物,特别是区分PLMS.
- 进一步研究肌细胞分化的途径可以改善肉瘤的诊断和分类.
更多相关视频
08:57Author Spotlight: Genetically Engineered Mouse Models and Pathological Characterization of Neurofibromatosis Type 1 Associated Tumors
Published on: May 17, 2024
2.1K
14:47Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
9.9K
相关概念视频
Formation of Muscle Fibers from Myoblasts
5.1K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.1K
Satellite Stem Cells and Muscular Dystrophy
2.0K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.0K
Smooth Muscle Contraction
3.8K
Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
3.8K
Structure and Organization of Smooth Muscles
6.1K
Smooth muscle tissue is a type of muscle tissue that can be found lining various vital organs in the human body, including the lungs, blood vessels, digestive tract, and respiratory tract. This type of tissue is responsible for regulating the movements of these organs, playing crucial roles in the functioning of various systems, including the vascular, digestive, respiratory, and urinary systems.
Structure of smooth muscle cell
Smooth muscle cells are spindle-shaped with tapering ends and a...
Structure of smooth muscle cell
Smooth muscle cells are spindle-shaped with tapering ends and a...
6.1K
Role of Myosin in Cell Migration
2.4K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
2.4K
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
