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在慢性淋巴细胞白血病和骨髓瘤中,HAT-PCR 能够对最小残留疾病进行敏感量化

Elizabeth Hughes1, Sue Latham1, Bryone Kuss1

  • 1School of Medicine, Flinders University, Adelaide 5042, Australia.

International journal of molecular sciences
|August 28, 2025
PubMed
概括

高A/T或高火温度PCR (HAT-PCR) 有效量化慢性淋巴细胞白血病和骨髓瘤的最小残留疾病 (MRD). 这种敏感的方法在淋巴细胞恶性瘤中为MRD检测提供了实际优势.

关键词:
进行PCR急性淋巴细胞白血病慢性淋巴细胞白血病数字PCR检测流细胞测量可测量的残留疾病最少残留疾病骨髓瘤下一代测序

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...