基于深度学习的成像分析揭示了辐射诱导的旁观者对癌细胞迁移和西斯普拉丁的调节的影响
Ryosuke Seino1, Hisanori Fukunaga1
1Department of Biomedical Science and Engineering, Faculty of Health Sciences, Hokkaido University, N12 W5 Kita-ku, Sapporo 060-0812, Japan.
International journal of molecular sciences
|August 28, 2025
概括
化学辐射疗法可以意外地增加癌细胞迁移,即使在非向区域. 这项研究使用HeLa-FUCCI细胞表明,虽然仅辐射会抑制迁移,但添加西斯会增强迁移,可能会恶化转移.
科学领域:
- 癌症学
- 细胞生物学
- 放射治疗研究
背景情况:
- 瘤侵袭和转移显著影响癌症患者的预后.
- 了解化疗对细胞运动的非向影响至关重要.
- 细胞迁移是瘤入侵和转移的一个关键过程.
研究的目的:
- 研究化疗对癌细胞迁移的影响,包括非向性影响.
- 使用宫癌细胞系分析辐射诱导的细胞运动变化.
- 确定化疗是否增强或抑制照射和非照射区域的细胞迁移.
主要方法:
- 使用HeLa-FUCCI细胞进行实时细胞周期阶段可视化.
- 应用块以创建不同的辐射 (场内) 和非辐射 (场外) 区域.
- 在暴露后24小时内进行时间延迟成像,使用或不使用西斯的2GyX射线.
- 使用Cellpose 2.0和TrackMate 7进行细胞细分和迁移跟踪分析.
主要成果:
- 仅仅辐射就显著降低了外场细胞的迁移速度和距离,表明旁观者抑制效应.
- 在cisplatin的存在下,In-field和Out-of-field细胞的迁移速度和总距离显著增加.
- 化学放射治疗,特别是使用西斯,似乎可以在直接辐射场之外增强瘤细胞的运动性.
结论:
- 化学放射治疗可能会产生意想不到的后果,可能会促进瘤细胞迁移和转移.
- 在放射治疗中添加西斯可能会无意中增加癌症扩散的风险.
- 这些发现强调了在癌症治疗策略中考虑非向效应的重要性,以改善患者的治疗结果.
更多相关视频
05:39Author Spotlight: Radiotherapy and Clonogenic Assays for Advancing Cancer Research and Personalized Medicine
Published on: April 5, 2024
949
05:11Author Spotlight: Establishing a Murine Non-Small Cell Lung Cancer Model for Developing Nanoformulations of Anticancer Drugs
Published on: May 10, 2024
1.2K
相关概念视频
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...