通过 lncRNA H19/miR-21-3p 调节以增强ABCG2表达来缓解Cichorium intybus L. 的药理机制和活性成分
在PubMed上查看摘要
概括
此摘要是机器生成的。奇科里提取物通过增强ATP结合卡塞特子家族G成员2 (ABCG2) 载体表达来缓解尿酸沉积. 它通过调节lncRNA H19/ miR-21-3p轴来实现这一目标,为健康提供新的治疗见解.
科学领域
- 肝脏病学
- 药理学
- 分子生物学
背景情况
- 由尿酸过引起的尿酸沉积导致脏炎症和损伤.
- Cichorium intybus L. () 传统上用于高尿血和痛风,但其对的影响尚不清楚.
- ATP结合盒子子子家族G成员2 (ABCG2) 载体和lncRNA H19/ miR-21-3p轴参与酸盐的结.
研究的目的
- 研究提取物对尿酸沉积的治疗作用和机制.
- 阐明ABCG2,IncRNA H19和miR-21-3p在尿酸沉积中的作用.
- 鉴定中的活性化合物,负责其治疗效果.
主要方法
- 在老鼠中诱导尿酸沉积.
- 评估了血清尿酸,尿酸沉积,炎症标志物,功能和组织学.
- 使用双酶测定,RT-qPCR,免疫组织化学,UPLC-LTQ-Orbitrap-MS和分子对接.
主要成果
- 在受影响的老鼠中,香草提取物逆转了血清尿酸升高,减少了尿酸沉积,并减少了炎症.
- 一个涉及lncRNA H19,miR-21-3p和ABCG2的调节轴被证实;香草调节了这个轴以调节ABCG2.
- 鉴定了69种西瓜成分,其中11β,13-二氨基基基因具有调节ABCG2和lncRNA H19/ miR-21-3p轴的潜力.
结论
- 奇科里提取物通过 lncRNA H19/ miR- 21-3p轴增强ABCG2表达,有效地减轻酸盐沉积.
- 通过lncRNA H19/ miR- 21-3p调节的ABCG2下调是尿酸沉积的关键病原机制.
- 11β,13-二氨基基可能是香草治疗效果的关键活性成分,提供了新的治疗策略.
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