紫外线衍生突变体Trichoderma sp 的细胞酶生产 浸泡发酵下的Mut-4:参数优化和大规模应用
Iksu Ha1,2, Seungjun Kim1, Yun-Yeong Lee1
1Department of Forest Products and Biotechnology, Kookmin University, Seoul 02707, Republic of Korea.
International journal of molecular sciences
|August 28, 2025
概括
对Trichoderma sp进行潜水发酵的优化 Mut-4显著增加了细胞酶的产生. 最好的条件提高了酶活性和生产力,显示出工业应用的潜力.
科学领域:
- 微生物学
- 生物技术
- 酶工程
背景情况:
- 浸泡发酵 (SmF) 对于工业酶生产至关重要.
- 优化培养条件是最大化酶产量的关键.
- 皮类动物 是一个众所周知的细胞酶生产者.
研究的目的:
- 优化介质配方和培养条件,以增强Trichoderma sp的细胞酶生产. 使用SMF的MUT-4.
- 评估碳源混合,源和初始pH对细胞酶活动和生产力的影响.
- 评估优化条件的可扩展性,从容器到反应器的规模.
主要方法:
- 使用一次因素 (OFAT) 方法进行参数优化.
- 研究了各种碳来源比率 (Avicel:纤维素),来源 (酵母提取物) 和初始pH值 (5.5).
- 细胞质生产和蛋白质度的比较.
主要成果:
- 确定了最佳条件:Avicel与纤维素的比例为 3:1,酵母提取物作为源,初始pH为5.5.
- 实现较早启动细胞酶的产生.
- 细胞酶成分活性增加了1. 17-1. 43倍 (瓶子) 和1. 3-2. 0倍 (反应器).
- 在两种尺度上显著提高蛋白质度.
结论:
- 优化的SmF参数显著提高了Trichoderma sp的细胞酶活性和生产力. 这是一个很好的选择.
- 这种优化的菌株显示出具有高成本效益的大规模细胞酶生产的潜力.
- 进一步的研究可以探索额外的优化策略以获得更高的产量.
更多相关视频
10:51High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
Published on: December 5, 2016
10.0K
06:08Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
368
相关概念视频
Bioreactor Controls-III
67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67
Upstream Processing
97
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
97
Production of Organic Acids
105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105
Production of Antibiotics
265
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
265
Production of Pharmaceuticals
94
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
94
Production of Biopesticides
108
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
108
