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Updated: Sep 10, 2025

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宫性微生物组和HPV:对16S/ITS NGS和微生物社区进行病毒协会分析的标准化方法

Jane Shen-Gunther¹, Qingqing Xia², Hong Cai³

¹Gynecologic Oncology & Molecular Medicine, Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.

International journal of molecular sciences

|August 28, 2025

概括

选择正确的16S rRNA高变区对于准确的宫和阴道微生物组概况至关重要. 这项研究强调不同区域如何影响结果,影响我们对阴道失调和HPV相关致癌的理解.

科学领域:

微生物学
基因组学
妇科瘤学

背景情况:

16S rRNA下一代测序 (NGS) 推动了宫和阴道微生物组的研究.
来自有限的16S超变区的方法偏差可能会影响结果.
了解这些偏差对于准确的分析和疾病关联研究至关重要.

研究的目的:

为16S/ITS NGS开发标准化的工作流程.
确定影响微生物丰度和分类特异性的方法偏差.
澄清高变性区域选择对宫菌群分析的影响.

主要方法:

使用商业NGS工具和16S/ITS QIAseq V1-V9选面板.
使用的ATCC阴道微生物标准和CLC基因组工作台与VAGIBIOTA数据库.
在66个宫细胞学样本中描述了微生物群落.

主要成果:

V3V4区域显示最小的定量偏差;V1V2提供最高的特异性.
微生物特征和社区状态类型 (CST) 与之前的研究保持一致.
在HSIL和HPV-16阳性样本中,缺氧性CST IV-B的病原体得到了丰富.

结论:

在16S rRNA超变区之间存在明显的物种识别差异.
区域选择对子宫环境中的微生物概况产生重大影响.
精确的区域选择对于了解HPV相关致癌的微生物作用至关重要.

关键词:

16S rRNA 的测序 ITS 测序生物信息学子宫癌人类乳头瘤病毒下一代测序分类学分类阴道微生物组

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