在谷物填充阶段在低温压力下具有不同耐寒度的米品种的转录动力学
Liangzi Cao1,2, Xueyang Wang3, Yingying Liu4
1Institute of Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, Harbin 150088, China.
Genes
|August 28, 2025
概括
低温会损害大米的品质, 但耐寒品种保持更好的味道和谷物含量. 粉代谢和转录因子中的关键基因对于谷物填充期间的耐寒性至关重要.
科学领域:
- 植物科学
- 分子生物学
- 农业科学
背景情况:
- 在填充米粒的过程中,低温应力显著降低了米粒的质量和产量.
- 发展耐寒品种对于维持受影响地区的农业生产力至关重要.
研究的目的:
- 研究大米在谷物填充阶段耐寒的生理和分子机制.
- 确定与冷反应相关的候选基因和转录因子,用于改良米品种的育种.
主要方法:
- 八种大米 (四种耐寒,四种耐寒) 在谷物填充过程中受到17.5°C的低温压力.
- 分析包括氨糖和蛋白质含量,味道质量和基因表达特征的RNA测序.
- 用权重基因共同表达网络分析 (WGCNA) 来识别关键的调节基因.
主要成果:
- 低温压力增加了所有品种的氨糖和蛋白质含量,但对寒冷敏感的品种显示了明显的味道质量下降.
- 在耐寒品种 (LD18,4-1021) 中,粉和糖代谢中的关键基因被上调,而在耐寒品种中被抑制.
- 鉴定了几种对冷反应的转录因子 (NAC,WRKY,AP2/ERF,MYB,bZIP家族) 和枢纽基因 (OsWRKY1,OsWRKY24,OsWRKY53,OsMYB4,OsPAL04,OsCDPK7).
结论:
- 这项研究阐明了在米粒填充过程中对低温应激的分子反应.
- 已识别的候选基因和转录因子为开发耐寒米的分子育种策略提供了基础.
更多相关视频
08:39Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
Published on: October 11, 2024
1.8K
05:22Transverse Sectioning of Mature Rice Oryza sativa L. Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
Published on: January 25, 2022
3.8K
相关概念视频
Responses to Heat and Cold Stress
13.8K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.8K
Responses to Drought and Flooding
10.9K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
10.9K
Gene Regulation During Sporulation
77
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
77
Stringent Response in E. coli
52
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
52
Transcription
148.1K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
148.1K
Translational Regulation
91
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
91
