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基于Aptamer-CRISPR/Cas12a的侧流技术可视化检测红肉中的内源性损伤因子 Neu5Gc
Yuxi Guo1, Honglin Ren1, Han Wang1
1State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Foods (Basel, Switzerland)
|August 28, 2025
概括
非红肉产品中的N-glycolylneuraminic酸 (Neu5Gc) 可能引发免疫反应和癌症风险. 一个新的aptamer-Cas12a侧流量测定可以快速地在现场检测Neu5Gc污染.
科学领域:
- 生物化学
- 食品科学
- 免疫学
背景情况:
- N - 甘氨基神经酸 (Neu5Gc) 是一种在红肉中发现的动物衍生分子.
- 人类和家禽中Neu5Gc的代谢可以引起免疫反应,炎症和增加癌症风险.
- 目前的Neu5Gc污染检测方法往往耗时,不适合现场分析.
研究的目的:
- 开发一种快速和具体的现场检测方法,以检测非红肉食品中的Neu5Gc污染.
- 使用Cas12a酶系统进行敏感的Neu5Gc检测.
- 为实际的食品安全应用创建横向流量测试.
主要方法:
- 开发一种基于aptamer的Neu5Gc识别系统.
- 通过免疫染色学技术整合aptamer-Cas12a的跨裂活性.
- 为视觉检测建立横向流量测试带测量.
主要成果:
- 开发的测定方法专门检测非红肉样本中的Neu5Gc污染.
- 在25分钟内实现检测,最小检测极限为10 ng/mL.
- 该试验成功地在添加的和实际的食品样本中检测出Neu5Gc.
结论:
- 亚普坦-Cas12a侧流测定为现场检测Neu5Gc提供了快速和灵敏的方法.
- 这项技术可用于确保非红肉食品,动物生物制品和家禽料的安全性.
- 该测试有助于预防与Neu5Gc暴露相关的潜在健康风险.
相关概念视频
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
01:28CRISPR/Cas9 Genome Editing
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...