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转谷氨酸酶修饰的乳清蛋白分离物-奶粉复合物的表征及其在Yuba膜中的应用
Junliang Chen1,2,3, Yao Chen1,2,3, Weiwei Cao1,2,3
1College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.
Foods (Basel, Switzerland)
|August 28, 2025
概括
转胺酶 (TGase) 通过修改乳清蛋白分离剂和大豆奶复合物来提高玉米的质量. 这种酶改善了蛋白质结构,增加了拉伸强度,减少了损失,以获得更好的产量和质地.
科学领域:
- 食品科学
- 蛋白质化学
- 生物技术
背景情况:
- 乳清蛋白分离物 (WPI) 和大豆奶是有价值的食品成分.
- 转质氨酶 (TGase) 酶促进蛋白质的交叉链接.
- 目前尚不清楚TGase对WPI-豆奶复合物的影响.
研究的目的:
- 研究TGas度对WPI-豆奶复合物质的影响.
- 评估TGase对WPI-豆奶复合物的物理化学和结构特性的影响.
- 确定TGase修饰对复合木的膜形成特性和质量的影响.
主要方法:
- 在不同度下使用TGase来修改WPI-豆奶复合物.
- 测量包括自由硫含量,自由氨基含量,颗粒大小和表面疏水性.
- 结构分析包括β片含量测定和扫描电子显微镜 (SEM).
- 对复合玉米的机械性能 (拉伸强度) 和损耗进行了评估.
主要成果:
- TGase 增加了 WPI- 豆奶复合物的颗粒大小,在低度下具有稳定性.
- TGase修饰改变了三级蛋白质结构,增加了β片含量.
- 表面疏水性降低,随着TGase的添加,自由的硫和氨基团减少.
- 复合玉米的拉伸强度达到0.05%的TGase,增加了7.8%.
- 经过TGase处理的玉米呈现出密集,无孔的结构和减少损失.
结论:
- TGase有效地改变了WPI-豆奶复合物的物理化学和结构性质.
- 这种处理提高了机械强度,并减少了复合玉米的损失.
- 这种酶改造提供了一种有前途的方法来改善传统的玉米产量和质量.
相关概念视频
01:02Protein Digestion
Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
02:41Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
01:57Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
02:41Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
01:57Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
01:12Protein Absorption
Proteins in the gastrointestinal tract typically come from food, but they can also originate from disintegrated cells or secreted enzymes. In the stomach, the enzyme pepsin breaks down these proteins into polypeptides. The fragments then move into the duodenum as a semi-fluid mass called chyme. Pancreatic proteases, such as trypsin and chymotrypsin, and intestinal brush border enzymes like carboxypeptidases further dismantle the polypeptides into tripeptides, dipeptides, and free amino acids.