牛摩扎雷拉乳清和瑞科塔消耗乳清的微生物分析:对潜在的益生菌主导菌株的洞察
Andrea Bonfanti1, Romano Silvestri2, Ettore Novellino3,4
1Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy.
Microorganisms
|August 28, 2025
概括
牛奶酪乳清和瑞可塔奶酪精制乳清含有大量的乳酸细菌和酵母. 某些Enterococcus菌株具有益生菌潜力,为食品生物技术和肠道健康提供价值.
科学领域:
- 乳制品科学
- 微生物学
- 食品生物技术
背景情况:
- 来自水牛肉和瑞科塔奶酪生产的乳清是有价值的副产品.
- 这些副产品的微生物群体的特征对于识别有益的微生物至关重要.
研究的目的:
- 描述水牛肉酪乳清 (CW) 和瑞科塔奶酪消耗乳清 (RCEW) 的微生物群落.
- 评估从这些乳清副产品中分离出的乳酸细菌 (LAB) 的益生菌潜力.
主要方法:
- 用于微生物群体分析的综合培养依赖和独立方法.
- 分析发现了主要的细菌和酵母物种.
- 基于培养的隔离发现了亚主导的LAB菌株.
- 根据耐酸性pH,胆盐,酶,生物膜形成和抗菌活性来评估益生菌的潜力.
主要成果:
- 转基因编码显示了LAB (例如Streptococcus thermophilus,Lactobacillus delbrueckii) 和耐热酵母 (例如Cyberlindnera jadinii) 的优势.
- 培养分离发现了Leuconostoc mesenteroides,Enterococcus faecalis和Enterococcus durans菌株.
- Enterococcus faecalis CW1和Enterococcus durans RCEW2对酸,胆盐和酶表现出耐受性,具有强大的生物膜形成和抗菌活性.
- 胆汁盐的耐药性表明可能对胆固醇代谢有好处.
结论:
- CW和RCEW是新型本土益生菌菌株的储存库.
- 这些乳制品副产品对食品生物技术和肠道健康应用具有重大潜力.
- 这项研究强调了区域乳制品副产品在开发功能成分方面的价值.
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