RcsB 和 H-NS 都有助于抑制csgDEFG操作子的表达
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
在PubMed上查看摘要
概括
此摘要是机器生成的。大肠杆菌中的RcsB调节器直接抑制生物膜形成基因csgD转录,并间接通过RprA小RNA降低其转化. 这种RcsBCD系统控制着膜的产生.
科学领域
- 微生物学
- 分子生物学
- 细菌遗传学
背景情况
- 在大肠杆菌中的生物膜形成中,Curli fimbriae是必要的.
- 卷状膜的表达受到转录因子和小调节性RNA (sRNA) 的严格调节.
- RcsD-RcsC-RcsB体系统参与外应力反应并调节膜表达.
研究的目的
- 研究RcsB调控器在csgD的转录控制中的作用,这是生物膜形成中的关键基因.
- 阐明RcsB影响csgD表达和膜产生的机制.
- 确定RcsB化对其DNA结合亲和力和调节功能的影响.
主要方法
- DNase-I足迹分析以确定csgD促进体上的RcsB结合位.
- 基因表达分析 (过度表达研究) 评估RcsB及其突变物对csgD表达的影响.
- 在不同的RcsB条件下评估形形成和RprAsRNA表达.
主要成果
- RcsB直接与csgD的促进子区域结合,导致转录抑制.
- 过度表达RcsB或非化突变物 (RcsB D56A) 减少了csgD表达和卷曲形成.
- RcsB积极调节RprAsRNA的表达,从而抑制csgD的翻译.
结论
- RcsBCD系统通过RcsB的直接转录抑制抑制csgD的表达.
- 通过RprAsRNA的诱导,RcsB还可以调解csgD的转化抑制.
- RcsB的DNA结合亲和力在很大程度上独立于其酸化状态.
相关概念视频
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...