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在SARS-CoV-2废水多重试验中N2和E基因度的变异性

Ashley Green¹, Aiswarya Rani Pappu¹, Melanie Oakes²

¹Department of Civil and Environmental Engineering, University of California Irvine, Irvine, CA 92697, USA.

|August 28, 2025

概括

原料选择对SARS-CoV-2废水监测产生影响. 在超过10%的样本中,包膜 (E) 基因的度高于N2基因,这凸显了在疾病监测中需要仔细选择基因的必要性.

科学领域:

环境微生物学
分子流行病学
公共卫生监督

背景情况:

基于废水的监测 (WBS) 追踪病原体并预测疾病的流行.
SARS-CoV-2 WBS通常使用核体 (N1,N2) 和包裹 (E) 基因标.
基于基因目标选择的结果变化需要调查.

研究的目的:

在多重RT-ddPCR试验中调查SARS-CoV-2 N2和E基因点之间的变异.
在COVID-19废水监测期间观察到基因目标性能差异的原因.

主要方法:

分析了2021年12月至2022年6月的700多个原始废水样本.
使用N2,E基因和小鼠肝炎病毒 (MHV) 的三重RT-ddPCR试验作为过程控制.
进行的水质测试 (TSS,COD,pH,度,NH3-N) 和多重回归分析.

主要成果:

在超过10%的样本中,E基因度超过了N2基因度.
较高的TSS和COD水平对病毒恢复产生了负面影响,表明潜在的PCR抑制.
水质变量或MHV恢复与基因度变异之间没有明确的关系.

结论:

原料选择对废水中SARS-CoV-2的量化有重大影响.
方法标准化对于可靠的大规模废水监测至关重要.
需要进一步的研究来优化精确的WBS基因标.

关键词:

美国其他类型人口洞开始回收效率基于废水的监测

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