使用Azohydromonas lata DSM 1123从Ricotta奶酪耗尽的乳清中生产PHB的基质优化
Angela Longo1, Luca Sconosciuto2, Michela Verni1
1Department of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy.
Microorganisms
|August 28, 2025
概括
使用*Azohydromonas lata*进行的瑞科塔奶酪乳清的价值化有效地产生了多基酸盐 (PHAs). 优化的基板和生物反应器条件显著提高了微生物生物质和PHA产量,证明了可持续的生物塑料生产方法.
科学领域:
- 生物技术和工业微生物学
- 聚合物科学
- 乳制品副产品的价值化
背景情况:
- 瑞科塔奶酪消耗乳清 (RCEW) 是全球广泛存在的乳制品副产品,对环境有重大影响.
- 价值化RCEW对于可持续性和经济效益至关重要,为微生物产品提供潜在的碳来源.
- 聚酸 (PHAs) 是可生物降解的聚合物,具有多种应用,但成本效益的生产仍然是一个挑战.
研究的目的:
- 通过*Azohydromonas lata* DSM 1123研究使用RCEW作为聚氨酸 (PHA) 生产基质的可行性.
- 优化RCEW预处理和发酵条件,以最大限度地提高微生物生物质和PHA产量.
- 描述产生的PHA聚合物.
主要方法:
- 包括乳糖,蛋白质和脂肪含量的RCEW组成的特征.
- 优化RCEW预处理,包括巴氏杀菌,杀菌和矿物质/酶补充剂 (β-galactosidase).
- 在生物反应器中使用*A. lata*在不同的条件下进行发酵试验 (限,C/N比率,食批量).
- 微生物生物质,细胞内PHA含量和PHA产量的分析.
- 提取的聚合物 (多基酸盐,PHB) 的热稳定性和分子量.
主要成果:
- RCEW含有显著的乳糖 (3. 81%),但需要β-银酸酶进行*A. lata*代谢.
- 优化RCEW预处理 (巴氏消毒混合物,矿物补充剂) 将生物质和PHA含量提高到25.94%.
- 在生物反应器中中度限 (C/N44) 和料批发发酵进一步增加了PHA的产量,达到0.88g/L (32.74%的细胞内积累).
- 产生的聚合物被确定为具有高热稳定性和5. 9KDa的聚酸 (PHB).
结论:
- 里科塔奶酪消耗乳清是使用*Azohydromonas lata*生产多酸 (PHA) 的可行且具有成本效益的基材.
- 优化基质预处理和发酵策略,包括料批处理,是最大限度地提高PHA产量的关键.
- 这项研究展示了乳制品废弃物回收和生物降解聚合物生产的可持续方法.
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