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通过抑制植物中的细菌生长,提高了胡中的细菌叶斑抵抗性

Mousami Poudel¹, Sophia McDuffee¹, Gerald V Minsavage¹

¹Department of Plant Pathology, University of Florida, Gainesville, FL 32611, USA.

Plants (Basel, Switzerland)

|August 28, 2025

概括

衰退性耐药性基因bs5,特别是与bs6或bs8结合使用时,可显著减少胡和西红中的细菌斑点病. 这些基因,特别是BS5的Pyramiding提供了对不断演变的Xanthomonas病原体的持久,广泛的抗药性.

科学领域:

植物病理学
分子遗传学
植物育种

背景情况:

胡 (BSP) 和西红 (BST) 的细菌斑点,由Xanthomonas spp引起,严重影响全球作物生产.
毒性种族的出现导致了主导性抵抗基因的分解 (例如,Bs2),需要持久的,非种族特异性的抵抗策略.
衰退性抗性基因 (*bs5*, *bs6*, *bs8*) 提供了基于非过敏反应的广泛抗性的潜力.

研究的目的:

系统地评估单个和组合的衰退性耐药基因 (*bs5*, *bs6*, *bs8*) 对关键的*Xanthomonas*物种的疗效.
评估这些基因对植物细菌种群增长和疾病发展的影响.
为培养胡品种对新生病原体的耐药性提供见解.

主要方法:

在易受感染的"Early Calwonder" (ECW) 背景下,携带不同组合的*bs5*,*bs6*和*bs8*的近同位素线 (NIL) 的发展.
在植物评估细菌种群大小和疾病症状严重程度.
对三种Xanthomonas物种进行测试:X. euvesicatoria (*Xe*),X. hortorum* pv. 它们是: *gardneri* (*Xhg*) 和 *X. perforans* (*Xp*)

主要成果:

*bs5*基因,特别是与*bs6*或*bs8*结合时,在所有测试的*Xanthomonas*物种中显著降低了细菌生长和疾病严重程度.
三重堆叠的线 (ECW568,携带*bs5*,*bs6*和*bs8*) 显示出最强的抑制病原体的扩散和疾病症状.
单独使用*bs6*和*bs8*或组合使用基本上无效,而与单独使用*bs8*相比,一些组合的效果有所降低.

结论:

衰退基因 *bs5* 在给予细菌斑点的定量耐药性方面发挥着核心作用.
金字塔式衰退性耐药性基因,特别是那些涉及*bs5*的基因,为持久的,广泛的疾病控制提供了附加效益.
这些发现支持使用衰退性耐药基因来培育改进的胡品种,并控制像*X. perforans*这样的进化细菌病原体.

关键词:

类植物细菌的叶子斑美国美国美国基因金字塔化衰退性阻力

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