消亡抑制效应蛋白 ChEC88 采用了在全属的 Hemibiotrophic 植物病原体中保存的新型结构动机
Shinya Ohki1, Hiroyuki Takahara2, Tomohiro Imamura2
1Center for Nano Materials and Technology (CNMT), Japan Advanced Institute of Science and Technology (JAIST), Nomi 923-1292, Ishikawa, Japan.
Plants (Basel, Switzerland)
|August 28, 2025
概括
植物病原菌使用保存的效应蛋白,如ChEC88,来抑制植物免疫力. 这些在各种真菌物种中发现的蛋白质具有相似的结构和功能,揭示了宿主免疫逃避的共同策略.
科学领域:
- 植物病理学
- 菌群学
- 分子生物学
背景情况:
- 植物病原菌使用效应蛋白来克服植物的防御,但它们的多样性使理解它们的作用变得复杂.
- 保存的效应器对于破译植物和真菌的分子相互作用至关重要.
研究的目的:
- 研究Colletotrichum higginsianum*中的效应蛋白ChEC88的功能和保存情况.
- 阐明其他植物病原菌中ChEC88及其同类的结构和功能关系.
主要方法:
- 核磁共振 (NMR) 光谱用于ChEC88的结构分析.
- 同类模型用于预测相关蛋白质的结构.
- 用于识别保存的同类物质的PSI-BLAST
- 功能性测试以评估ChEC88和同类药物抑制NLP1诱导的植物细胞死亡的能力.
- 位点定向的突变发生,以确定关键的功能残留物.
主要成果:
- ChEC88表现出一种新的伪双重对称的3D结构.
- ChEC88及其同类,包括来自*Pyricularia oryzae*的BAS3,有效地抑制了由和乙烯诱导的类蛋白1 (NLP1) 诱导的植物细胞死亡.
- 在*Colletotrichum*,*P. oryzae*和*Fusarium*物种中保留了ChEC88的同类物种.
- 确定了对ChEC88免疫抑制功能的特定残留物.
结论:
- 双生菌植物病原菌利用一种涉及ChEC88类蛋白质的保存免疫抑制机制.
- 这些效应物很可能是从共同的祖先血统进化出来的,这表明了宿主操纵的共同进化策略.
更多相关视频
10:07Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
24.1K
09:15Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
Published on: October 20, 2022
2.4K
相关概念视频
Necrosis
4.8K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
4.8K
Notch Signaling Pathway
4.4K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.4K
Viral Structure
63.5K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
63.5K
The Intrinsic Apoptotic Pathway
6.8K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.8K
The Extrinsic Apoptotic Pathway
6.6K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.6K
Chemotaxis in E. coli
95
Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
95
