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相关概念视频

Hydrolysis of ATP01:08

Hydrolysis of ATP

The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids.
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine monophosphate—by the removal of a second...

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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
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微流体ATPS器件的细胞分区设计:使用冠状细胞和模型微粒的动态能量策略和计算

Gabriel Garibaldi1, Jimena Alegria1, Anita Shayan1

  • 1Department of Biomedical Engineering and Chemical Engineering, The University of Texas at San Antonio, San Antonio, TX 78249, USA.

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概括

这项研究引入了微流体水性双相系统 (ATPS) 的新能源平衡模型,以分离细胞的分区动力学. 这种方法可以有效地分离不同的细胞表型,用于生物医学应用.

关键词:
水性双相系统 (ATPS)细胞分离人类冠状细胞微流体表面能量

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相关实验视频

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科学领域:

  • 生物医学工程
  • 细胞生物学
  • 分离科学

背景情况:

  • 细胞分类对于药物发现和诊断至关重要.
  • 像FACS和MACS这样的传统方法在吞吐量和成本方面存在局限性.
  • 水性双相系统 (ATPS) 提供了一个有前途的替代方案,特别是微流体.

研究的目的:

  • 开发微流体ATPS设备的合理设计策略.
  • 为了分离具有相似起源但不同表型的细胞.
  • 克服设计ATPS微妙细胞分离的挑战.

主要方法:

  • 在PEG-DextranATPS模型中对影响细胞分裂的材料特性进行系统的描述.
  • 开发一种考虑界面能量和粘性散射的能量平衡方法.
  • 在ATPS分区过程中估计细胞接口转位动态.

主要成果:

  • 在ATPS接口完成细胞分区的时间被确定为关键分离参数.
  • 一个能量平衡模型被开发并通过实验测量得到验证.
  • 这项研究表明,基于分区动态,有可能分离健康和患病的人类淋巴细胞.

结论:

  • 动态能源方法为优化微流体ATPS设备提供了基础.
  • 这种策略可以有效地分离表型相似的细胞群.
  • 这些发现扩大了微流体细胞分离的生物医学应用潜力.